Choice of Differentiation Media Significantly Impacts Cell Lineage and Response to CFTR Modulators in Fully Differentiated Primary Cultures of Cystic Fibrosis Human Airway Epithelial Cells
Abstract: In vitro cultures of primary human airway epithelial cells (hAECs) grown at air–liquid interface have become a valuable tool to study airway biology under normal and pathologic conditions, and for drug discovery in lung diseases such as cystic fibrosis (CF). An increasing number of different differentiation media, are now available, making comparison of data between studies difficult. Here, we investigated the impact of two common differentiation media on phenotypic, transcriptomic, and physiological features of CF and non-CF epithelia.
qPCR primer design revisited
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative detection of amplification targets
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
The complexity of such
analysis usually makes this a time-consuming and very difficult task for users, especially when the primers have a
large number of hits. Furthermore, although the BLAST program has been widely used for primer target detection,
it is in fact not an ideal tool for this purpose as BLAST is a local alignment algorithm and does not necessarily
return complete match information over the entire primer range.
The coagulation system in host defense
The blood coagulation system and immune system of higher organisms are thought to have a common ancestral origin. During infections, the blood coagulation system is activated and components of the hemostatic system are directly involved in the immuneresponse and immune system modulations. The current view is that the activation of
coagulation is beneficial for infections with bacteria and viruses.
